Cardiogen

Cardiogen is the synthetic tetrapeptide Ala-Glu-Asp-Arg (AEDR), a cardiovascular-oriented member of the Khavinson short peptide series. Researchers have tested the Cardiogen peptide in organotypic myocardial cultures from young and old rats, in embryonic fibroblasts where it changed cytoskeletal protein levels, and in a transplanted sarcoma model in senescent rats. Its arginine C-terminus distinguishes it from the related AED-based peptides. Research Market supplies Cardiogen as a lyophilized research reagent, with purity and identity data for each lot reported on its certificate of analysis. It is sold strictly for laboratory research use.

Chemical structure and identification

• Molecular formula: C₁₈H₃₁N₇O₉
• Molecular weight: 489.5 g/mol
• CAS number: 857267-11-9
• PubChem CID: 11583989
• Amino acid sequence: Ala-Glu-Asp-Arg (AEDR)
• Synonyms: H-Ala-Glu-Asp-Arg-OH
• Form: Lyophilized powder
• Analytical methods: RP-HPLC for purity and mass spectrometry for identity, reported on the batch certificate of analysis

Research applications

• Myocardial tissue culture: At 10^-12 M in organotypic myocardium explants from 3- and 24-month-old rats, cardiogen stimulated cell proliferation and lowered p53 expression [1]
• Cytoskeleton and nuclear matrix: In cultured mouse embryonic fibroblasts, H-Ala-Glu-Asp-Arg-OH raised actin, tubulin and vimentin 2- to 5-fold and lamin A and C 2- to 3-fold [2]
• Tumor vasculature model: In senescent rats with transplanted M-1 sarcoma, cardiogen increased tumor cell apoptosis and slowed tumor growth through hemorrhagic necrosis, without a direct cytostatic effect [3]
• Prostate fibroblasts: In aging cultures of human prostate fibroblasts, cardiogen and two other short peptides raised CXCL12, WEDC1 and ghrelin expression [4]
• Cardiovascular senescence (review): A review of the senescence-associated secretory phenotype in cardiovascular cells discusses AEDR among peptides that regulate inflammaging-related molecules [5]

Reference citations

1. Chalisova N. et al. (2009). [The effect of the amino acids and cardiogen on the development of myocard tissue culture from young and old rats]. Adv Gerontol, 22(3):409–413. PMID 20210190
2. Khavinson V. et al. (2012). Tetrapeptide H-Ala-Glu-Asp-Arg-OH stimulates expression of cytoskeletal and nuclear matrix proteins. Bull Exp Biol Med, 153(4):559–562. PMID 22977870 · DOI
3. Levdik N. et al. (2009). Tumor-modifying effect of cardiogen peptide on M-1 sarcoma in senescent rats. Bull Exp Biol Med, 148(3):433–436. PMID 20396706 · DOI
4. Kheĭfets O. et al. (2010). [Peptidergic regulation of the expression of signal factors of fibroblast differentiation in the human prostate gland in cell aging]. Adv Gerontol, 23(1):68–70. PMID 20586252
5. Khavinson V. et al. (2022). Senescence-Associated Secretory Phenotype of Cardiovascular System Cells and Inflammaging: Perspectives of Peptide Regulation. Cells, 12(1):106. PMID 36611900 · DOI

Compound data from PubChem CID 11583989. Literature summaries describe published preclinical research and are not claims about this product’s effects in humans or animals.