Bronchogen

Bronchogen is the synthetic tetrapeptide Ala-Glu-Asp-Leu (AEDL), a respiratory-focused member of the Khavinson peptide series. The main in-vivo work is a rat model of chronic obstructive lung pathology induced by nitrogen dioxide, where bronchial epithelium structure and lavage fluid markers were measured. Biophysical papers on the Bronchogen peptide examine its binding to DNA and its effect on DNA melting temperature. Research Market supplies Bronchogen as a lyophilized research reagent, with purity and identity data for each lot reported on its certificate of analysis. It is sold strictly for laboratory research use.

Chemical structure and identification

• Molecular formula: C₁₈H₃₀N₄O₉
• Molecular weight: 446.5 g/mol
• CAS number: 857267-12-0
• PubChem CID: 11690869
• Amino acid sequence: Ala-Glu-Asp-Leu (AEDL)
• Form: Lyophilized powder
• Analytical methods: RP-HPLC for purity and mass spectrometry for identity, reported on the batch certificate of analysis

Research applications

• Obstructive lung model: In rats exposed to NO₂ for 60 days, a month of bronchogen was reported to eliminate goblet cell hyperplasia, squamous metaplasia and loss of ciliated cells in bronchial epithelium and raised secretory IgA [1]
• Lavage fluid markers: In the same NO₂ rat model, bronchogen lowered neutrophilic inflammation in bronchoalveolar lavage and increased secretory IgA and surfactant protein B [2]
• Bronchial epithelial differentiation: In human embryonic bronchial epithelial cell cultures, bronchogen stimulated expression of the differentiation factors CXCL12 and Hoxa3, more so in aged cultures [3]
• DNA sequence preference: Fluorescence quenching assays indicated that bronchogen binds preferentially to deoxyribooligonucleotides with CNG sequences, in particular CTG [4]
• DNA thermostability: Differential scanning microcalorimetry showed bronchogen raising the melting temperature of calf thymus and mouse liver DNA by 3.1 °C over a narrow peptide/DNA ratio range [5]

Reference citations

1. Kuzubova N. et al. (2015). Modulating Effect of Peptide Therapy on the Morphofunctional State of Bronchial Epithelium in Rats with Obstructive Lung Pathology. Bull Exp Biol Med, 159(5):685–688. PMID 26468022 · DOI
2. Titova O. et al. (2017). [Antiinflammatory and regenerative effect of peptide therapy in the model of obstructive lung pathology]. Ross Fiziol Zh Im I M Sechenova, 103(2):201–208. PMID 30199201
3. Khavinson V. et al. (2012). Peptides tissue-specifically stimulate cell differentiation during their aging. Bull Exp Biol Med, 153(1):148–151. PMID 22808515 · DOI
4. Fedoreyeva L. et al. (2011). Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry (Mosc), 76(11):1210–1219. PMID 22117547 · DOI
5. Monaselidze J. et al. (2011). Effect of the peptide bronchogen (Ala-Asp-Glu-Leu) on DNA thermostability. Bull Exp Biol Med, 150(3):375–377. PMID 21240358 · DOI

Compound data from PubChem CID 11690869. Literature summaries describe published preclinical research and are not claims about this product’s effects in humans or animals.