5-AMINO-1MQ

5-Amino-1MQ spray provides the NNMT inhibitor 5-amino-1-methylquinolinium in solution in a spray bottle. Nicotinamide N-methyltransferase connects nicotinamide, a NAD+ precursor, to S-adenosylmethionine-dependent methylation; its expression rises in white adipose tissue and liver of obese mice and with age in skeletal muscle. That makes NNMT inhibition a research tool in studies of aged-mouse muscle regeneration and strength, and of the gut microbiome in diet-induced obese mice. Research Market supplies the 5-Amino-1MQ spray with identity and purity data for each lot on its certificate of analysis. It is sold strictly for laboratory research use.

Chemical structure and identification

• Molecular formula: C₁₀H₁₁IN₂
• Molecular weight: 286.11 g/mol
• CAS number: 42464-96-0
• PubChem CID: 66522933
• Active cation: 5-amino-1-methylquinolinium, C₁₀H₁₁N₂⁺, 159.21 g/mol (PubChem CID 950107)
• Synonyms: 5-amino-1-methylquinolinium iodide, NNMTi
• Form: Supplied in solution in a spray bottle

Research applications

• Aged muscle stem cells: In 24-month-old mice with barium chloride injury to the tibialis anterior, a small-molecule NNMT inhibitor increased muscle stem cell proliferation and fusion, myofiber cross-sectional area and peak torque; in C2C12 myoblasts it promoted differentiation [1]
• Muscle function in aged mice: Mice treated from 22 to 24 months of age with an NNMT inhibitor, intensive exercise or both were compared for grip strength, running capacity, fiber size and intramyocellular lipid, with proteome and metabolome profiling of muscle [2]
• Gut microbiome: In diet-induced obese mice switched to a low-fat diet, 5-amino-1-methylquinolinium treatment was associated with a distinct cecal microbiome pattern, including lower Erysipelatoclostridium and higher Lactobacillus abundance than vehicle [3]
• NNMT in adipose tissue: NNMT expression was increased in white adipose tissue and liver of obese and diabetic mice, and NNMT inhibition raised adipose SAM and NAD⁺ levels [4]

Reference citations

1. Neelakantan H. et al. (2019). Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle. Biochem Pharmacol, 163:481–492. PMID 30753815 · DOI
2. Dimet-Wiley A.L. et al. (2024). Nicotinamide N-methyltransferase inhibition mimics and boosts exercise-mediated improvements in muscle function in aged mice. Sci Rep, 14(1):15554. PMID 38969654 · DOI
3. Dimet-Wiley A. et al. (2022). Reduced calorie diet combined with NNMT inhibition establishes a distinct microbiome in DIO mice. Sci Rep, 12(1):484. PMID 35013352 · DOI
4. Kraus D. et al. (2014). Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature, 508(7495):258–262. PMID 24717514 · DOI

Compound data from PubChem CID 66522933. Literature summaries describe published preclinical research and are not claims about this product’s effects in humans or animals.